Until recently, the prostate biopsy, and accompanying trans-rectal ultrasound, has been the domain of senior doctors. However, there is no reason why a senior, appropriately trained nurse could not undertake this part of the diagnostic pathway. This article describes the development of a one-stop, nurse-led prostate cancer clinic. It shows how the entire diagnostic pathway for men with suspected prostate cancer can be nurse-led in a safe and competent manner. Continuous robust audit of the patient experience and of safety issues relating to the biopsy itself describe a service that is welcomed by patients, that has dramatically improved their experience, has significantly improved waiting times and is as safe as a medically-led service.